Fig 7.

 Colocalization staining of apoptotic nuclei and Hsp70 expression in epithelial cells from medullary collecting ducts. Effect of Losartan on LP. Through colocalization staining of apoptotic nuclei (TUNEL technique) with HSP70 expression, apoptotic nuclei appeared as intense brown-staining nuclei and cytoplasmic slight brown HSP70 expression in the same tubule epithelial cells from medullary collecting ducts. Light counterstaining with 0.5% with hematoxylin was performed to reveal nonapoptotic nuclei, nuclei appeared as blue staining. Increased Hsp70 expression and decreased apoptosis induction after Losartan administration in LP. (A) Histological sections of kidney medulla from NP and NP plus Losartan fed rats: Lower number of apoptotic cells were seen in OMCDs and IMCDs (sloping arrow). Slight Hsp70 staining was shown (left pointing arrow). Non apoptotic nuclei appeared as blue staining (up arrow). (B) Histological sections of kidney medulla from NP and NP plus Losartan fed rats: Lower number of apoptotic cells were seen in OMCDs and IMCDs (right pointing arrow). Slight Hsp70 staining was shown (lower right pointing arrow). Nonapoptotic nuclei appeared as blue staining (down arrow). (C) Histological section of kidney medulla from LP fed rats. Higher number of apoptotic nuclei (upper arrow), with weak Hsp70 immunoreaction (lower arrow) were shown in the same epithelial cells from MCDs. (D) Histological section of kidney medulla from LP plus Losartan fed rats: Through colocalization staining, few apoptotic nuclei associated with increased cytoplasm immunoreaction for Hsp70 (right pointing arrow) was demonstrated in cytoplasm of OMCD in the same epithelial cells. Nonapoptotic nuclei appeared as blue staining (up arrow). Magnification, 630×. The colors mentioned in the text refer to the original specimens