Fig 9.

 Western Blot analysis for 32-kDa procaspase 3 protein and caspase 3 activity in kidney medulla from NP, LP, and RP for 14, 21, and 30 days. Top, Total protein (50 μg) was extracted from cytosol, and equal amounts of protein were loaded and separated by molecular weight on 12% SDS-polyacrylamide gel electrophoresis (PAGE). Blot represents 1 of 5 separate experiments. Bottom, Caspase 3 activity was assessed by level of Ac-DEVD-AMC cleavage release of fluorescence AMC tag. Activity is expressed as picomoles of AMC/minute/microgram of protein. LP compared with NP (***P < 0.001), RP for 14 days vs NP 14 days (**P < 0.01). Caspase 3 activity and procaspase 3 protein assay data were obtained from the same 5 independent samples