Fig 4.

 p53 protein is up-regulated in CCRF-CEM but not SW480 cells expressing Hsp70. (A) Expression of p53 and the p53-regulated genes Bax and MDM2 in parental, vector control, and Hsp70-expressing cells was assessed by Western blotting. (B) Expression of p53 mRNA was assessed by SYBR Green RTQPCR, and relative expression was determined by comparison with vimentin. (C) The effect of Hsp70 on expression of p53 in SW480 cells was evaluated by Western blotting. (D) Expression of TRAIL-R1 and TRAIL-R2 on the plasma membrane of SW480 cells was analyzed by flow cytometry using specific antibodies