Fig 5.

 Enhanced p53 protein in the presence of elevated Hsp70 increases binding to the TRAIL-R2 gene. Chromatin from fixed and sonicated cell lysates from untreated CCRF-CEM, vector control, and Hsp70-expressing cells was immunoprecipitated with a human p53-specific antibody. Immunoprecipitated DNA was analyzed by SYBR Green RTQPCR performed in triplicate using primers specific to the p53 binding region or to a nonspecific site 900 bp upstream of the consensus site. The experiment was repeated twice with similar results each time. Statistical analysis by Student's t-test; *P < 0.05 compared to vector control; **P < 0.05 clone 7 vs clone 16