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. 1972 Apr;10(4):649–659.

Thymus origin of lymphocytes reacting and stimulating reaction in mixed lymphocyte cultures-studies in the rat

B P Maclaurin
PMCID: PMC1713118  PMID: 5034246

Abstract

Mixed lymphocyte cultures between pairs of allogeneic cell suspensions from rat thymus of one strain versus thymus, lymph node, spleen or bone marrow from a histo-incompatible strain, have shown a decreasing gradient of stimulation capacity. After complete neonatal thymectomy 6 weeks previously, lymph-node lymphocytes are shown to have diminished or absent capacity to stimulate allogeneic rat thymocytes in mixed culture. It it concluded that thymus-derived lymphocytes appear to be required both for stimulation as well as for reaction in the mixed lymphocyte test.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amos D. B., Bach F. H. Phenotypic expressions of the major histocompatibility locus in man (HL-A): leukocyte antigens and mixed leukocyte culture reactivity. J Exp Med. 1968 Oct 1;128(4):623–637. doi: 10.1084/jem.128.4.623. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BAIN B., VAS M. R., LOWENSTEIN L. THE DEVELOPMENT OF LARGE IMMATURE MONONUCLEAR CELLS IN MIXED LEUKOCYTE CULTURES. Blood. 1964 Jan;23:108–116. [PubMed] [Google Scholar]
  3. ELKINS W. L. INVASION AND DESTRUCTION OF HOMOLOGOUS KIDNEY BY LOCALLY INOCULATED LYMPHOID CELLS. J Exp Med. 1964 Sep 1;120:329–348. doi: 10.1084/jem.120.3.329. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Guttmann R. D., Lindquist R. R., Ockner S. A. Renal transplantation in the inbred rat. IX. Hematopoietic origin of an immunogenic stimulus of rejection. Transplantation. 1969 Oct;8(4):472–484. doi: 10.1097/00007890-196910000-00019. [DOI] [PubMed] [Google Scholar]
  5. Hardy D. A., Ling N. R. Effects of some cellular antigens on lymphocytes and the nature of the mixed lymphocyte reaction. Nature. 1969 Feb 8;221(5180):545–548. doi: 10.1038/221545a0. [DOI] [PubMed] [Google Scholar]
  6. Hardy D. A., Ling N. R., Knight S. C. Exceptional lymphocyte stimulating capacity of cells from lymphoid cell lines. Nature. 1969 Aug 2;223(5205):511–512. doi: 10.1038/223511a0. [DOI] [PubMed] [Google Scholar]
  7. Heslop B. F. Histocompatibility antigens in the rat: the AS2 strain in relation to the AS, BS and HS strains. Aust J Exp Biol Med Sci. 1968 Aug;46(4):479–491. doi: 10.1038/icb.1968.41. [DOI] [PubMed] [Google Scholar]
  8. Heslop B. F. Maturation of histocompatibility antigens and host reactivity in the rat. Transplant Proc. 1969 Mar;1(1):560–563. [PubMed] [Google Scholar]
  9. Iversen J. G. Specific antisera against recirculating and non-recirculating lymphocytes in the rat. Clin Exp Immunol. 1970 Jan;6(1):101–108. [PMC free article] [PubMed] [Google Scholar]
  10. Kisken W. A., Swenson N. A. Unresponsiveness of mixed leucocyte cultures from thymectomized adult dogs. Nature. 1969 Oct 4;224(5214):76–77. doi: 10.1038/224076a0. [DOI] [PubMed] [Google Scholar]
  11. Knight S., Ling N. R. A comparison of the responses of lymphocytes from blood, spleen, thymus and appendix of rabbits of different ages to stimulation "in vitro" with staphylococcal filtrate. Clin Exp Immunol. 1969 Jun;4(6):667–684. [PMC free article] [PubMed] [Google Scholar]
  12. REIF A. E., ALLEN J. M. THE AKR THYMIC ANTIGEN AND ITS DISTRIBUTION IN LEUKEMIAS AND NERVOUS TISSUES. J Exp Med. 1964 Sep 1;120:413–433. doi: 10.1084/jem.120.3.413. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Raff M. Theta isoantigen as a marker of thymus-derived lymphocytes in mice. Nature. 1969 Oct 25;224(5217):378–379. doi: 10.1038/224378a0. [DOI] [PubMed] [Google Scholar]
  14. Roitt I. M., Greaves M. F., Torrigiani G., Brostoff J., Playfair J. H. The cellular basis of immunological responses. A synthesis of some current views. Lancet. 1969 Aug 16;2(7616):367–371. doi: 10.1016/s0140-6736(69)92712-3. [DOI] [PubMed] [Google Scholar]
  15. Schellekens P. T., Vriesendorp B., Eijsvoogel V. P., Van Leeuwen A., Van Rood J. J., Miggiano V., Ceppellini R. Lymphocyte transformation in vitro. II. Mixed lymphocyte culture in relation to leucocyte antigens. Clin Exp Immunol. 1970 Feb;6(2):241–254. [PMC free article] [PubMed] [Google Scholar]
  16. Schwarz M. R. The mixed lymphocyte reaction: an in vitro test for tolerance. J Exp Med. 1968 May 1;127(5):879–890. doi: 10.1084/jem.127.5.879. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Schwarz M. R. Transformation of rat small lymphocytes with allogeneic lymphoid cells. Am J Anat. 1967 Nov;121(3):559–570. doi: 10.1002/aja.1001210308. [DOI] [PubMed] [Google Scholar]
  18. Silvers W. K., Wilson D. B., Palm J. Mixed leukocyte reactions and histocompatibility in rats. Science. 1967 Feb 10;155(3763):703–704. doi: 10.1126/science.155.3763.703. [DOI] [PubMed] [Google Scholar]
  19. Steel C. M., Hardy D. A. Evidence of altered antigenicity in cultured lymphoid cells from patients with infectious mononucleosis. Lancet. 1970 Jun 20;760(1):1322–1323. doi: 10.1016/s0140-6736(70)91913-6. [DOI] [PubMed] [Google Scholar]
  20. Wilson D. B., Blyth JL NOWELL P. C. Quantitative studies on the mixed lymphocyte interaction in rats. 3. Kinetics of the response. J Exp Med. 1968 Nov 1;128(5):1157–1181. doi: 10.1084/jem.128.5.1157. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Wilson D. B. Quantitative studies on the mixed lymphocyte interaction in rats. I. Conditions and parameters of response. J Exp Med. 1967 Oct 1;126(4):625–654. doi: 10.1084/jem.126.4.625. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Wilson D. B., Silvers W. K., Nowell P. C. Quantitative studies on the mixed lymphocyte interaction in rats. II. Relationship of the proliferative response to the immunologic status of the donors. J Exp Med. 1967 Oct 1;126(4):655–665. doi: 10.1084/jem.126.4.655. [DOI] [PMC free article] [PubMed] [Google Scholar]

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