Table 1.
Phenotype of tph1 embryos depends on maternal genotype
| Maternal genotype | Paternal genotype | No. of crosses | Embryo's genotype |
% of small and/or abnormal embryos | ||
|---|---|---|---|---|---|---|
| +/+ | +/− | −/− | ||||
| +/+ | +/+ | 3 | 28 | — | — | 3.6* |
| +/− | 1 | 4 | 6 | — | 0 | |
| −/− | 2 | — | 27 | — | 3.7* | |
| +/− | +/+ | 1 | 6 | 4 | — | 0 |
| +/− | 4 | 11 | 24 | 10 | 6.7* | |
| −/− | 2 | — | 4 | 3 | 0 | |
| −/− | +/+ | 3 | — | 10 | — | 80† |
| +/− | 1 | — | 9 | 9 | 88.9† | |
| −/− | 2 | — | — | 15 | 86.7† | |
Data show the type (maternal and paternal genotypes) and number of crosses performed to produced the embryos and the number of embryos of wild-type, heterozygous, and homozygous null genotype. All 170 embryos were measured; 104 embryos were further analyzed.
*A total of 3.6–6.7% of embryos obtained from tph1+/+ or tph1+/− females were normal but with smaller CRL (7.4–7.5 mm).
†A total of 80–88.9% of embryos from tph1−/− mothers were smaller (CRL: 5.8–7.4 mm) and presented developmental abnormalities. The remaining 15–20% were normal in size albeit the CRL values were in the lower range. Sagittal cuts presented no gross abnormalities.