Results of co-localization assay. 1, 3, and 5 (black columns) are samples for both beta-actin and Arp2 mRNAs. 2, 4 and 6 (white columns) are samples for only beta-actin mRNA but processed with the second TSA for residual HRP activity of the first TSA. Between the two TSA, cell samples were treated with PBS (1–2); NaN3/H2O2 (3–4), or HCl (5–6). Significant difference between each treatment group (P < 0.01). Error bar = SEM.