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. 2003 Aug 15;112(4):598–607. doi: 10.1172/JCI17129

Figure 6.

Figure 6

IFN-γ and IL-6 modulate leukocyte apoptosis during SES-induced peritoneal inflammation. Annexin V/PI staining of leukocytes isolated from the peritoneal cavity of wild-type, IFN-γ–/–, and IL-6–/– mice. Representative scatter plots show FACS analysis of gated PMN obtained from mice stimulated with SES for 6 hours. Apoptotic cells were identified according to annexin V+/PI– (lower right quadrant, early apoptosis) and annexin V+/PI+ (upper right quadrant, late apoptosis/necrosis) staining. The proportion of cells residing in each quadrant is expressed as a percentage (mean ± SEM, n = 4–7 mice/condition; *P < 0.05, a significant increase versus IFN-γ–/– plus SES). By comparison with values from wild-type mice, mean reductions of 40.2% and 55.4% were respectively seen in early and late apoptotic events for IFN-γ–/– mice and of 66.2% and 48.9% for IL-6–/– mice. The proportion of annexin V+/PI– PMN in mouse peripheral blood was always less than 1% (data not shown).