Figure 1.

mRNA expression of Cats V, L, and S. (a) Real-time RT-PCR analysis for the expression of Cats V, L, and S in thymus (T, black bars) and PBMCs (gray bars). The graphs display the averaged values of seven thymi and five PBMC samples. (b) Cat V in the thymus is exclusively found in an epithelial cell-enriched fraction (Ep) and could not be detected in thymocytes (Th). (c and d) Laser capture microdissection of the thymic medulla (Me, black bar) and cortex (Co, gray bar), followed by RT-PCR analysis shows that Cat V is mostly found in the cortex of the thymus.