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In vitro efficacy of free and HPMA copolymer-bound doxorubicin. The cytotoxicity of free doxorubicin, poly(HPMA)-GFLG-doxorubicin, and control copolymer (lacking doxorubicin) was assessed by investigating the ability of agents to inhibit the colony formation of AT1 rat prostate carcinoma cells. Values represent the average ± SD of three independent experiments.
