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. 2006 Nov;8(11):917–924. doi: 10.1593/neo.06364

Figure 3.

Figure 3

Confirmation of Tiam1 expression in different clones by real-time PCR and Western blot analysis. (A) Quantification of Tiam1 mRNA expression in clones transduced by the pLenti6/Tiam1 (site B) lentivirus relative to controls (clone transduced by pLenti6/U6 lentivirus). (B) Western blot analysis shows marked reduction of Tiam1 protein expression in clones 2 and 8. Lane 1, SW480/EGFP cells; lane 2, pLenti6/Tiam1 lentivirus-transduced clone 2; lane 3, pLenti6/Tiam1 lentivirus-transduced clone 8; lane 4, pLenti6/Tiam1 lentivirus-transduced clone 4; lane 5, pLenti6/U6 lentivirus-transduced clone.