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Derivation of anoikis-resistant variants of IEC-18 cells. A. Experimental protocol (see text). B. Morphology of spheroids composed of IEC-18, AR1.10, AR2.10 or ras-3 cells after 48 hours in 3-dimensional culture. (Original magnification x 100) C. Cumulative survival curves (MTS assay) of anoikis resistant variants of IEC-18 cells derived by spheroid selection protocol (AR1.10, AR2.10) or transformation with mutant H-ras oncogene (ras-4).
