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. 1999 Apr;1(1):80–89. doi: 10.1038/sj.neo.7900010

Figure 3.

Figure 3

RT-PCR analysis of p73 mRNA in neuroblastoma cell lines. Agarose gel electrophoresis of p73 cDNA fragments amplified with different primer pairs. A. After the first round of RT-PCR amplification with primer set 3 (see Figure 1), PCR products were detected in 8 of 18 cell lines, including one single-copy MYCN cell line (SJNB1) and 7 MYCN-amplified lines (SJNB 5, 6, 8, 14, 17, 18, and 20). B. After nested PCR with primer set c, products were detected in 14 of the cell lines, but not in SJNB4, 12, 13, and 16. C. After a first round of RT-PCR with primer set 5 (see Figure 1) and nested PCR with primer set e, 6 different products were observed. The expected sizes of each of the bands are indicated on the right side of the figure. D. Primers specific for β-actin mRNA were used to amplify products from each cell line, demonstrating RNA integrity. The first and last lanes of each gel show the mobilities of markers consisting of a 100-bp ladder.