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. 1999 Apr;1(1):42–49. doi: 10.1038/sj.neo.7900004

Figure 1.

Figure 1

Representative gel of melanocytic specimens for telomerase activity: benign lesions (blue nevi, compound melanocytic nevi, junctional melanocytic nevi); malignant lesions (primary melanoma Clark II, Clark III, and Clark IV); benign lymph node, metastatic lymph node, and a distal subcutaneous metastatic melanoma. Negative control: lysis buffer; positive telomerase controls (a human breast epithelial cell line immortalized with human papilloma virus 16E6/E7): 10 cells, 100 cells, and 1000 telomerase-positive cells. ITAS, a 150-bp fragment containing TS and CX sequences at the ends, co-amplifies with the telomerase ladder. It is included for semiquantitative telomerase activity level analyses and to detect PCR or Taq polymerase inhibitors.