Figure 5.
Bcl-XL-Δloop but not Bcl-XL is resistant to cleavage by caspases and is able to inhibit apoptosis in the short- and long-term assays. Jurkat cells overexpressing Bcl-XL or Bcl-XL-Δloop were irradiated with 10 Gy of ionizing radiation, and at various times cells were collected for the preparation of cell extracts for Western blot analysis with a Bcl-XL-specific antibody (A). At these times cells were also collected for determination of cell viability with trypan blue exclusion (B). Control cultures that were mock irradiated were also analyzed in parallel. Data presented in (B) are an average of 4 experiments, with bars representing standard error.