Fig. 1.
Localization of the Syt binding site in BoNT/B and BoNT/G. (A) Computer-based surface analysis of potential Syt binding sites in HCC of BoNT/B. The electrostatic Connolly surface of the HCC of BoNT/B (Protein Data Bank entry 1F31) is displayed. Blue, hydrophilic area; green, hydrophobic area; white, neutral; red, surface exposed. Blue dots represent the recognition of four suitable pockets by the SuperStar algorithm, whereas gray and red balls indicate polar and hydrophobic interaction points found by Site Finder. (B) Residues forming the Syt binding site (pocket 4) of HCC of BoNT/B are shown as sticks. (C) Shown is the homolog Syt binding site of the HCC of BoNT/G. Carbon atoms in orange indicate residues subjected to mutational analysis. The highlighted tryptophan in B and C indicates the location of the ganglioside binding site.