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. 2006 Dec 26;3(12):e467. doi: 10.1371/journal.pmed.0030467

Figure 1. Validation Analyses of Gene Expression Profiling.

Figure 1

(A) QRT-PCR validations of several candidate survival-related genes. Bars represent fold changes for the selected genes with differential expression between long- (>5 y) and short-term survival (<2 y) patients. Positive fold change represents up-regulated, and negative fold change represents down-regulated in short-term survival patients. * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.005.

(B and C) Immunostaining analysis of CRABP1 and ABCC1 expression in long- and short- term survival lung cancer patients. Low magnification (B) and 40× (C). Positive CRABP1 immunoreactivity was observed in cytoplasm of an acinar ADC (lower left photomicrographs of B and C) from short-term survival patients, and no CRABP1 reactivity was seen in a lung ADC from a long-term survival patient (upper left). Strong ABCC1 membranous staining (lower right) in tumor cells from short-term survival patients was observed, and weak ABCC1 reactivity was seen in a lung ADC from a long-term survival patient (upper right).

(D) Distribution of CRABP1 and ABCC1 protein levels in short- and long-term survival patients.