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. 2007 Jan 2;117(1):153–164. doi: 10.1172/JCI29154

Figure 2. Hypertriglyceridemia in Ndst1-deficient mice.

Figure 2

Total triglycerides (A) and total cholesterol (B) were measured in plasma samples (n = 20 per genotype, mixed males and females, horizontal bars indicate mean values). Triglyceride (C) and cholesterol (D) content of lipoproteins fractionated by preparative density ultracentrifugation was determined. Each value is from 1 set of pooled plasma derived from 10 mice. Lipoproteins were also analyzed by FPLC gel filtration, and the amount of triglyceride (E) and cholesterol (F) was determined in control mice (filled circles) and Ndst1f/fAlbCre+ mice (open circles). Plasma was pooled from 9 mice of each genotype. The elution positions of human VLDL, IDL/LDL, and HDL are indicated. (G) Samples of purified lipoproteins (d < 1.019 g/ml) were analyzed by gradient SDS-PAGE, and the individual apolipoproteins were visualized by Coomassie blue or silver staining. The location of apoB-48, apoB-100, apoE, and apoAI was determined by Western blotting (data not shown). The location of apoCs and serum albumin was deduced by Mr values.