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. 2007 Jan 2;117(1):153–164. doi: 10.1172/JCI29154

Figure 5. Decreased plasma clearance and liver uptake of VLDL in Ndst1-deficient mice.

Figure 5

(A) Plasma clearance of human VLDL apoB-100 was measured by ELISA using human apoB-100–specific monoclonal antibody MB47 (see Methods). Control mice (filled squares; t1/2 = 23.2 ± 1.4 minutes; n = 6) and Ndst1f/fAlbCre+ mice (open squares; t1/2 = 46.6 ± 5.3 minutes; n = 6). The difference in t1/2 between the genotypes was significant (P = 0.0016), and the difference between the 10- and 20-minute time points for the mutant was also significant (P = 0.0133). Results were verified in a separate experiment. (B) Deconvolution microscopy showing uptake of DiI-VLDL (red) in the liver of Ldlr–/–Ndst1f/fAlbCre– and Ldlr–/–Ndst1f/fAlbCre+ mice. DiI-VLDL (100 μg) was injected by tail vein, and livers were fixed 20 minutes later. Sections of 20 μm were DAPI-stained for nuclei (blue). H, hepatocyte cords; S, sinusoids. Scale bar: 26 μm; magnification, ×40. (C) Hepatocytes were isolated from mice of the indicated genotypes and incubated with DiI-VLDL (5 μg/ml). Images were obtained by deconvolution microscopy.