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. 2007 Jan 2;117(1):175–184. doi: 10.1172/JCI29881

Figure 6. IL-10 prevents the effects of TNF-α on adipocytes.

Figure 6

(A) IL-10 decreases MCP-1 secretion by adipocytes. 3T3-L1 adipocytes were treated with media with or without IL-10 (20 ng/ml) for 16 hours. Conditioned media was then removed and assayed for MCP-1 levels by ELISA. n = 5 independent samples per condition. Data are expressed as mean ± SD. (B) IL-10 protects adipocytes from TNF-α–induced downregulation of insulin receptor and glucose transporter 4 (GLUT4) expression. 3T3-L1 cells were treated with or without IL-10 for 24 hours prior to treatment with or without TNF-α (17 ng/ml for 6 hours). Lysates were prepared and immunoblots probed with antibodies against the insulin receptor (IR) and GLUT4. (C) IL-10 maintains IRS levels despite treatment with TNF-α. Adipocytes were treated as described for B and lysates examined for IRS1 tyrosine phosphorylation induced by insulin (INS; 100 nM for 5 minutes) after immunoprecipitation of IRS1. IRS serine phosphorylation at Ser307 was evaluated using specific antibodies.