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. 2006 Dec 26;5(1):e6. doi: 10.1371/journal.pbio.0050006

Figure 5. Nab4 and Alternative 3′ Pre-mRNA Processing Plays a Role during Copper Stress.

Figure 5

(A) Serial dilutions of NAB4 and nab4-7 strains on plates containing 0, 0.5, and 1 mM CuSO4. Cells were grown at room temperature which is the permissive temperature for the nab4-7 strain.

(B) 3′ RACE of the CTR2 transcript during a shift to 37 °C of NAB4 and nab4-7 strains. Samples were taken at 0, 45, 75, and 120 min after shifting both strains to 37 °C, the non-permissive temperature of the nab4-7 strain. The RACE analysis displays three isoforms which correlate with the presence of three Nab4 motifs in the CTR2 3′ UTR. 100-base pair molecular weight markers are shown in lane 1 with the 300-base pair ladder visible at the bottom of the gel.

(C) Serial dilutions of the wild-type NAB4, the single mutant nab4-7, the single mutant Δctr2, and the double mutant nab4-7 Δctr2 strains. The plating conditions are 23 °C, 37 °C, 1 M CuSO4, and 0.25 M CuSO4. WT, wild-type.