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SDS/PAGE gels of wild-type and mutant HMMs. Purified HMM samples (A: wild type; B: W441F; and C: W512F) were subjected to SDS/PAGE on 8% and 15% discontinuous acrylamide gels. Gels were stained with Coomassie brilliant blue. HC, HMM heavy chain; ELC, essential light chain; RLC, regulatory light chain.
