Figure 1.

Schematic representation of the produced chimeras and wild-type H⁺,K⁺-ATPase. The open bars represent H⁺,K⁺-ATPase sequences, and the solid bars represent Na⁺,K⁺-ATPase sequences. HK, H⁺,K⁺-ATPase; NaK, Na⁺,K⁺-ATPase; HNN3, H⁺,K⁺-ATPase with amino acids Met1-Ile293 replaced by those of Na⁺,K⁺-ATPase (Met1-Ile279); HN36, H⁺,K⁺-ATPase with amino acids Ile293-Arg846 replaced by those of Na⁺,K⁺-ATPase (Ile279-Arg832); HN34, H⁺,K⁺-ATPase with amino acids Ile293-Leu346 replaced by those of Na⁺,K⁺-ATPase (Ile279-Leu332); HN56, H⁺,K⁺-ATPase with amino acids Leu776-Arg846 replaced by those of Na⁺,K⁺-ATPase (Leu762-Arg832); HN34/56, H⁺,K⁺-ATPase with amino acids Ile293-Leu346 and Leu776-Arg846 replaced by those of Na⁺,K⁺-ATPase (Ile279-Leu332 and Leu762-Arg832).