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Bivalent binding of 12G5 to MLV–CXCR4. Sensorgrams of 12G5 binding to MLV–CXCR4 are shown at different mAb concentrations, with the signals obtained from 12G5 binding to the MLV–pCDNA3 surface being subtracted. Binding was measured for 90 s and dissociation for 80 s before regeneration. Global analysis of the data using Biaevaluation 3.0 software was performed, and the boxes indicate the best fit of the data to a bimolecular interaction.
