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. 2000 Oct 3;97(21):11227–11231. doi: 10.1073/pnas.200361997

Figure 4.

Figure 4

Titration of MucB in translesion replication in the presence of MucA′, RecA, and SSB. The translesion replication assay was performed as described under Materials and Methods, with 2.5 μM MucA′, for 5 min. The concentrations of MucB were as follows: lane 3, 50 nM; lane 4, 100 nM; lane 5, 150 nM; lane 6, 200 nM; lane 7, 250 nM; lane 8, 300 nM. Lane 1 is a control without proteins, whereas lane 2 contains products of a reaction promoted by pol V (250 nM) and UmuD′ (2.5 μM) in the presence of RecA and SSB.