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MucA cannot substitute for MucA′ in MucB-promoted lesion bypass. The translesion replication assay was performed as described under Materials and Methods, for the indicated periods of time, with 250 nM MucB and 4 μM MucA′ or the indicated concentrations of MucA. Reaction products were restricted and analyzed by urea-PAGE followed by phosphorimaging. M, size marker for the 47-mer bypass product.
