Figure 1.

Generation of Ng KO mice. (A) Restriction map of the native and mutant Ng alleles and targeting vector. The locations of the Ng gene exon (solid block), herpes simplex virus thymidine kinase gene (hsv-TK), neomycin resistance gene (neo), lacZ gene, and the probe for Southern blot analysis to identify the various genotypes are shown. (B) Southern blot analysis of SacI-digested genomic DNA from mouse tails. (C) PCR analysis of the tail DNA for routine genotyping. (D) Northern blot analysis of Ng mRNA derived from olfactory bulb (OB), cerebral cortex (CR), and cerebellum (CB). Approximately 20 μg of RNA from each tissue was analyzed by using labeled rat Ng cDNA as a probe and referenced with signal probed with cyclophilin (cyc) cDNA. (E) Immunoblot analysis of Ng and Nm in tissue extracts derived from hippocampus and cerebral cortex. The polyclonal antibody (no. 270) detects both Ng and Nm.