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. 2000 Oct 3;97(21):11244–11249. doi: 10.1073/pnas.200139397

Figure 3.

Figure 3

Stimulation of binding of L-731,988 to FLAG-integrase by HIV-1 donor DNA. (A) FLAG-integrase associated with SPA beads was incubated with labeled L-731,988 titrated in the absence (●) or presence (■) of 100 nM U5 DNA. (B) FLAG-integrase associated with SPA beads was incubated with labeled L-731,988 (35 nM) and unlabeled L-731,988 titrated in the absence (●) or presence (■) of 100 nM U5 DNA. FLAG-Asp116Ala associated with SPA beads was incubated with labeled L-731,988 and a titration of unlabeled L-731,988 in the presence of 100 nM U5 DNA (×). (C) FLAG-integrase associated with SPA beads was incubated with L-731,988 with a titration of U5 viral ends (■, n = 7), a nonspecific DNA containing a 2-bp 3′ overhang (×, n = 4), or a disintegration dumbbell (●, n = 2). Averaged results of multiple experiments are shown and the standard error is indicated.