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. 2000 Oct 10;97(21):11292–11296. doi: 10.1073/pnas.97.21.11292

Figure 1.

Figure 1

Distinct activities of the CAD and KID in CREB in phosphorylation-dependent transcription activation in vivo and in vitro. (a) CAD, KID, and DNA-binding domain each contain amino acids 1–8 of CREB plus the indicated CREB amino acid sequence fused to amino acids 4–94 of the Gal4 DNA-binding domain. (b) JEG3 cells were cotransfected with the 5XGT-Luciferase reporter plasmid plus expression plasmids for CREB-Gal4 proteins ± the catalytic subunit of PKA. The data represent the means ± SEM of five independent experiments. (c) A representative experiment shows the effects of PKA titration on in vitro transcription mediated by CRG or S133A. (d) Western blots of purified CREB-GAL4 proteins, incubated ∓ 1 unit of PKA, were performed with anti-CREB (Upper) or anti-P-CREB (Lower) antisera.