Figure 4.

Phosphorylation of CRG stimulates multiple-round transcription. (a) A representative in vitro transcription experiment for multiple-round transcription is shown (from the same experiment illustrating single-round transcription in Fig. 2a). (b) The results (means ± SEM) of four experiments are shown. (c) The number of cycles of transcription was estimated by dividing the multiple-round value (Fig. 4b) by the single-round value (Fig. 2b) for each protein. The results (means ± SEM) of four experiments are presented. These experiments and those in Fig. 2 were done in parallel with the same template, nuclear extract, and CREB-Gal4 proteins.