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. 2000 Oct 10;97(21):11297–11302. doi: 10.1073/pnas.97.21.11297

Figure 1.

Figure 1

HIV-1-based gene transfer systems. (A) Helper (packaging) construct. The triangle symbolizes a deletion affecting the packaging signal between the 5′ splice donor site and the beginning of the gag sequence. The poly(A) site was derived from the bovine growth hormone gene. (B) Transducing vector constructs. The HIV-EGFP/HSA (i) and HIV-VP22 EGFP/HSA (ii) constructs are shown. Boxes interrupted by jagged lines contain partial deletions. CMV, Human CMV-IE promoter. (C) Env expression construct encoding VSV-G. VSV-G expression is driven by the HIV-1 LTR. The poly(A) site was derived from the simian virus 40 late region.