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FACS analysis of HOS cells infected with lentiviral vectors encoding VP22-EGFP fusion proteins. Approximately 2 × 105 cells were plated into six-well plates and infected with 0.25 ml of virus. Three days after infection the cells were detached from the plate and were then subjected to FACS analysis. (A) Mock-infected HOS-cells. (B) HOS cells infected by using the HIV-VP22-EGFP/HSA vector lacking viral Env. (C) HOS cells infected by using the HIV-VP22-EGFP/HSA vector.
