Figure 5.

In situ analysis of PCAF and PCAF-B/GCN5 mRNAs in mouse embryos. Dark-field micrographs of parasagittal sections of E10.5 (B–E) and E12.5 (G and H) mouse embryos after hybridization with a PCAF-specific antisense probe (B and E), a PCAF-B/GCN5-specific antisense probe (B and G), or the respective sense probes as a control (C and E). Bright-field micrographs of toluidine blue-stained sections of E10.5 (A) and E12.5 (F) are also shown. Abbreviations: fb, forebrain; h, heart; hb, hindbrain; hl, hind limb; li, liver; lu, lung; nt; neural tube; skm, skeletal muscle. (Bar = 1 mm.)