Phylogeny derived from sequence alignment of 2,404 bp of combined mtDNA
sequences from the control region homologous with the hypervariable
region 1 region in humans, COII and cytochrome b. Clades
are color-coded to emphasize species diversity. Individuals are
identified by unique laboratory extraction number (Yoder Lab
Extraction; YLE) and by locality. Distance tree was generated in
paup* 4.0b4a (PPC) (53) by using HKY85 correction model
(54) and weighted least squares (power = 2) algorithm. A total of
1,000 replicates of the random addition option were executed without
branch swapping. TBR branch swapping then was performed on best tree
(hit 399 of 1,000 trials). A single tree of score 0.83 (%SD =
3.27) resulted from the search and is shown with midpoint rooting.
Location of midpoint root was confirmed by multiple outgroup rootings.
Clade resolution and hierarchy is congruent with strict consensus of 12
trees derived from maximum parsimony analysis in which 100,000 random
additions were performed without branch swapping, followed by TBR
branch swapping of the 10 best trees. Numbers on branches indicate
statistical support from 100 bootstrap replicates with one random
addition per replicate. Propithecus,
Varecia, and Eulemur were used to root
the bootstrap tree. Circled numbers highlight bootstrap support for two
primary clades. Asterisks beside species designations indicate species
that have been reported to occur in sympatry with another mouse lemur
type. See ref. 11 for details.