Figure 2.

Coimmunoprecipitation of endogenous WRN with p50. Extracts enriched in nuclear and nucleolar proteins were prepared from HeLa cells, SV40-transformed normal human fibroblasts (WI-38), or fibroblast derived from Werner syndrome patients (WS). Aliquots containing approximately 800 μg protein were immunoprecipitated (IP) with a monoclonal antibody against the human WRN protein (WRN) or with normal mouse IgG as control (C). Western blot analysis was performed using anti-p50 (Upper, lanes 1–6) and anti-WRN antibodies (lanes 5 and 6, indicated separately). The lower panel indicates immunoblotting of the respective unprocessed extracts using anti-p50 antibody (input, lanes 1–6).