Immune regulation of serine protease relative AgISPR5.
Mosquitoes were subjected to the indicated experimental treatment, and
a Northern blot of poly(A)+ RNA from 20 mosquitoes per lane
was probed with AgISPR5. Samples were untreated (Control),
3-day antibiotic treatment (Antibiotic), 6 h after inoculation
with sterile saline (Injury), 6 h after inoculation with LPS
(LPS), 24 h after taking a normal uninfected bloodmeal (Normal),
and 24 h after a P. berghei-infective bloodmeal
(Infective). (Inset) Northern blot lanes in the same
order probed with AgISPR5 (Upper; 1.9 kb) and
rpS7 loading control (Lower; 0.7 kb).