Figure 5.

Immune regulation of putative protease inhibitor AgIMcr14. Northern blot of total RNA isolated from 15 mosquitoes per lane after the indicated experimental treatment, probed with AgIMcr14. Mosquitoes were untreated (Control), 6 h and 24 h after thoracic wounding with a sterile needle (Injury), 6 h and 24 h after thoracic wounding with bacterial culture (Bacteria), 24 h after taking a normal uninfected bloodmeal (Normal), and 24 h after a P. berghei-infective bloodmeal (Infective). (Inset) Northern blot lanes in the same order probed with AgIMcr14 (Top; 4.4 kb), loading control rpS7 (Middle; 0.7 kb), and AgIR28 (Bottom; 1.4 kb).