Table 1.
Immune-responsive A. gambiae genes, isolated from enriched cDNA library
| Clone | -Fold upregulated* | mRNA size, kb* | cDNA sequence | Sequence homologies† | GenBank accession no. |
|---|---|---|---|---|---|
| AgISerpF1 | 4 | 1.8 | Frag | Protease inhibitor, serpin family, E = e–21 | AF203339 |
| AgIMcr14 | 5‡ | 4.4‡ | Frag | Protease inhibitor, α2-macroglobulin and complement C3, E = e–9 | AF203333 |
| AgISPR1 | 30 | 1.4 | Frag | Serine protease, chymotrypsin-like, E = e–26 | AF203336 |
| AgISPR5 | 13 | 1.9 | Full | Clip-domain serine protease, blood coagulation factor X, E = e–14 | AF203334 |
| AgISPR9 | 3 | 2.2 | Frag | Serine protease, Limulus hemocyte factor D, E = e–21 | AF203337 |
| AgISPR10 | 100 | 2.5 | Frag | Serine protease, trypsin-like, E = e–2 | AF203338 |
| AgISPR20 | 10 | 1.5 | Frag | Serine protease, Limulus hemocyte factor D, E = e–5 | AF203335 |
| AgIR2 | 2 | 1.5 | Frag | None | AF283260 |
| AgIR4 | 5 | 1.4 | Full | None | AF283261 |
| AgIR6 | 8 | ND | Frag | (DNA damage-induced protein) | AF283262 |
| AgIR7 | 4 | 1.1 | Full | p10/OS, E = e–37 | AF283263 |
| AgIR15 | 19 | 1.9 | Frag | Nuclear scaffold attachment factor A, E = e–1 | AF283264 |
| AgIHEXB | 3 | 1.9 | Frag | β-hexosaminidase, β chain, E = e–11 | AF283265 |
| AgIR28 | 100 | 1.4 | Full | (DNA polymerase/homeodomain protein) | AF283266 |
| AgIR29 | 5 | 0.7 | Frag | None | AF283267 |
| AgIrpS18 | 1.5 | 1.0 | Frag | Ribosomal protein S18, E = e–6 | AF283268 |
| AgIrpS26 | 4 | 0.8 | Frag | Ribosomal protein S26, E = e–13 | AF283269 |
| AgIBB1 | 7 | 1.8 | Frag | None | AF283270 |
| AgICC4 | 6 | 2.2 | Frag | None | AF283271 |
| AgICE1 | 2 | 1.5 | Frag | None | AF283272 |
| AgIPAH | 9 | 1.8 | Frag | Phenylalanine hydroxylase, E = e–11 | AF283273 |
| AgIDB12 | 12 | 0.7 | Frag | A. gambiae ce5, E = e–20; (anti-thrombin) | AF283274 |
| AgIsHSP | 7 | 0.7 | Frag | Small heat shock protein, hsp20 family, E = e–31 | AF283275 |
Full, full length cDNA sequence; Frag, cDNA fragment, 300–600 bp; ND, no data.
*
Based on virtual Northern blots from LPS-inoculated A. gambiae, except where indicated.
†
Based on blast homology comparisons. E indicates the order of magnitude of the best “Expect” value from a blastp sequence comparison to the nonredundant peptide database, where scores <0.05 are considered significant. Entries in parentheses were weaker and speculative.
‡
Based on Northern blots from mRNA of bacteria-inoculated A. gambiae.