Skip to main content
Antimicrobial Agents and Chemotherapy logoLink to Antimicrobial Agents and Chemotherapy
. 1988 Jan;32(1):125–127. doi: 10.1128/aac.32.1.125

Radiometric method for pyrazinamide susceptibility testing of Mycobacterium tuberculosis in egg-yolk-enriched BACTEC 12A medium.

C L Woodley 1, R W Smithwick 1
PMCID: PMC172111  PMID: 3126704

Abstract

A radiometric method for testing the susceptibility of Mycobacterium tuberculosis to pyrazinamide in egg-yolk-enriched 12A medium (pH 5.5) is described. We obtained 100% agreement between the 7H10 agar method with 25 microgram of pyrazinamide per ml and the modified radiometric method with a drug concentration of 50 microgram/ml in tests of 90 clinical isolates.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Butler W. R., Kilburn J. O. Improved method for testing susceptibility of Mycobacterium tuberculosis to pyrazinamide. J Clin Microbiol. 1982 Dec;16(6):1106–1109. doi: 10.1128/jcm.16.6.1106-1109.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. CANETTI G., FROMAN S., GROSSET J., HAUDUROY P., LANGEROVA M., MAHLER H. T., MEISSNER G., MITCHISON D. A., SULA L. MYCOBACTERIA: LABORATORY METHODS FOR TESTING DRUG SENSITIVITY AND RESISTANCE. Bull World Health Organ. 1963;29:565–578. [PMC free article] [PubMed] [Google Scholar]
  3. Crowle A. J., Sbarbaro J. A., May M. H. Inhibition by pyrazinamide of tubercle bacilli within cultured human macrophages. Am Rev Respir Dis. 1986 Nov;134(5):1052–1055. doi: 10.1164/arrd.1986.134.5.1052. [DOI] [PubMed] [Google Scholar]
  4. EGGERTH A. H. Growth of Mycobacterium tuberculosis in egg yolk mediums. Proc Soc Exp Biol Med. 1950 Apr;73(4):542–545. doi: 10.3181/00379727-73-17738. [DOI] [PubMed] [Google Scholar]
  5. Heifets L. B., Iseman M. D. Radiometric method for testing susceptibility of mycobacteria to pyrazinamide in 7H12 broth. J Clin Microbiol. 1985 Feb;21(2):200–204. doi: 10.1128/jcm.21.2.200-204.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. McDERMOTT W., TOMPSETT R. Activation of pyrazinamide and nicotinamide in acidic environments in vitro. Am Rev Tuberc. 1954 Oct;70(4):748–754. doi: 10.1164/art.1954.70.4.748. [DOI] [PubMed] [Google Scholar]
  7. NORMAN J. O., WILLIAMS R. P. The use of egg-yolk dialysate for the growth of Mycobacterium tuberculosis. Am Rev Respir Dis. 1960 Mar;81:423–425. doi: 10.1164/arrd.1960.81.3.423. [DOI] [PubMed] [Google Scholar]
  8. Stottmeier K. D., Beam R. E., Kubica G. P. Determination of drug susceptibility of mycobacteria to pyrazinamide in 7H10 agar. Am Rev Respir Dis. 1967 Nov;96(5):1072–1075. doi: 10.1164/arrd.1967.96.5.1072. [DOI] [PubMed] [Google Scholar]
  9. Tarrand J. J., Gröschel D. H. Evaluation of the BACTEC radiometric method for detection of 1% resistant populations of Mycobacterium tuberculosis. J Clin Microbiol. 1985 Jun;21(6):941–946. doi: 10.1128/jcm.21.6.941-946.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Tarrand J. J., Spicer A. D., Gröschel D. H. Evaluation of a radiometric method for pyrazinamide susceptibility testing of Mycobacterium tuberculosis. Antimicrob Agents Chemother. 1986 Dec;30(6):852–855. doi: 10.1128/aac.30.6.852. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Woodley C. L. Evaluation of streptomycin and ethambutol concentrations for susceptibility testing of Mycobacterium tuberculosis by radiometric and conventional procedures. J Clin Microbiol. 1986 Feb;23(2):385–386. doi: 10.1128/jcm.23.2.385-386.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Antimicrobial Agents and Chemotherapy are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES