Figure 3.

RelB enhances MHC class I-restricted peptide presentation to a CD8⁺ CTL line. The CTL line LC13 is specific for the peptide sequence FLRGRAYGL, restricted by HLA-B8. (A) BL30 were transfected with either RelB or pCDNA3 and incubated for 24 h. Nonviable cells were removed by density-gradient centrifugation, and varying numbers of cells from each transfected population were added to 10⁴ LC13 in the presence or absence of 1 μg peptide. hIFN-γ levels were measured in the supernatant by ELISA after 24 h. (B) As above, 24 h after transfection, varying numbers of RelB or pCDNA3-transfected BL30 stably expressing FLRGRAYGL with or without endoplasmic reticulum signal sequence were added to 10⁴ LC13, and hIFN-γ was measured after 24 h. Data are the mean of triplicate wells and are representative of three separate experiments.