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Augmented viral clearance in the IL-15Tg mouse against the vaccinia virus. 2 × 108 plaque-forming units of the wt vaccinia virus were injected into five wt and IL-15Tg(K2) mice, and their blood was collected at the indicated time points after infection. After removing the plasma, blood cells were lysed by repeated freeze–thaw cycles, and the lysates were incubated with CV-1 cells for 36 h to monitor plaque formation.
