Figure 4  .

Immunostaining for VEGF receptors in non-vascularised ERMs is visualised with a red reaction product. (A) Flt-1 staining in a PVR ERM. (B) Preincubation of the primary antibody with the peptide against which it was generated eliminates most immunostaining in the same ERM shown in (A). (C) Flt-1 positivity is demonstrated in an idiopathic ERM. (D) Flk-1 immunostaining in a subretinal membrane from a patient with PVR. (E) Flk-1 immunostaining in a PVR ERM. (F) Flk-1 immunostaining in an idiopathic ERM. (HistoMark Red/haematoxylin; A, B, C, D, F ×145, E ×70).