Skip to main content
. 2000 Oct 3;97(21):11460–11465. doi: 10.1073/pnas.210384197

Figure 2.

Figure 2

I-Ak exclusively binds extended HEL(48-61) peptides through one single register. (A) A Lys52 P1 anchor inhibited the binding of HEL(48-61), blocking register 1. Prevention of register 1 binding with Lys52 substitutions inhibited the binding of the extended HEL(48-61) peptides containing three additional putative P1 anchors. (B) The register 1-specific antibody, Aw3.18, was used to immunoprecipitate I-Ak complexes individually formed with radiolabeled HEL(40-61), HEL(44-61), or HEL(48-61) peptides. Despite the presence of four potential binding registers, the Aw3.18 antibody equally immunoprecipitated I-Ak complexes with the extended HEL peptides. Consequently, I-Ak bound the extended HEL(40-61) and HEL(44-61) peptides through the same single register as HEL(48-61).