Table 1.

Proliferation and cytokine secretion by whole lymphocyte populations, from brains of Cop 1 immunized mice, with and without EAE induction

EAE challenge	Antigen	Proliferation, cpm	Cytokines, pg/ml
			IL-2	IFN-γ	IL-4	IL-5	IL-6	IL-10
+	−	183	−	2,169	−	−	568	4
+	Cop 1	406	53	2,166	9	−	944	64
+	MBP	198	−	1,850	−	−	653	42
+	Con A	582	458	6,012	38	−	961	14
−	−	104	−	136	−	−	73	−
−	Cop 1	301	−	143	29	123	154	165
−	MBP	126	−	134	−	−	86	77
−	Con A	499	235	264	31	123	104	41

(SJL × BALB/c)F₁ mice that had been rendered resistant to EAE by injection of Cop 1 (10 mg per mouse in ICFA) were challenged or unchallenged for disease, 3 weeks later, with spinal cord homogenate. One month after immunization with Cop 1, mice were perfused intracardially and brains were harvested. The isolated lymphocytes were evaluated for their reactivity by measuring proliferation response by thymidine incorporation in triplicate cultures, and cytokine secretion by quantitative ELISA in duplicates. Reactivity to medium alone, Cop 1 50 μg/ml, MBP 100 μg/ml, and Con A 1 μg/ml, was tested. Standard deviations were under 20% of the mean. Results represent one of four independent isolation experiments.