Figure 4  .

Distribution and composition of glycosaminoglycans. Keratan and dermatan sulphate were localised on cross sections of corneas 42 days after treated discs were inserted. (A), (D) Polybutylene disc inserted; (B), (E) polyester disc inserted; and (C), (F) ePTFE disc inserted. (A), (B), (C) Corneas stained with anti-dermatan sulphate shows dermatan sulphate within the disc. (D), (E), (F) Corneas stained with anti-keratan sulphate shows keratan sulphate within the disc (100×). Composition of glycosaminoglycans present 12 weeks after implantation of the porous materials. Aliquots from purified extracts were assayed for dermatan sulphate (DS), keratan sulphate (KS), and heparan sulphate (HS). Dermatan sulphate was defined as GAG susceptible to chondroitinase ABC. Keratan sulphate was defined as GAG susceptible to heparanase III. Data are presented as specific GAG/total GAG.