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. 2000 Oct 10;97(21):11482–11487. doi: 10.1073/pnas.97.21.11482

Figure 3.

Figure 3

Consumption of nitrogen oxide species at baseline, during NO synthase inhibition, and during exercise with NO synthase inhibition. Arterial and venous nitrite and S-nitrosothiol gradients were multiplied by flow measurements to determine consumption (positive value on the y axis) or, in the case of HMW-SNO, production (negative value on the y axis). SNO-Hb: Basal SNO-Hb consumption was 92 ± 86 pmol/ml/min/100 ml of tissue. SNO-Hb consumption increased nonsignificantly from 143 ± 83 pmol/ml/min/100 ml of tissue during L-NMMA infusion to 366 ± 471 pmol/ml/min/100 ml of tissue during L-NMMA infusion with exercise. Data are expressed as the mean ± SEM. HMW-SNO: At baseline, HMW-SNO forms (negative value for consumption) in the peripheral circulation at a rate of 23 ± 14 pmol/ml/min/100 ml of tissue. Although there was no significant change in the formation of HMW-SNO during L-NMMA infusion (25 ± 8 pmol/ml/min/100 ml of tissue), a trend toward increased formation of HMW-SNO was observed during exercise and L-NMMA infusion (127 ± 117 pmol/ml/min/100 ml of tissue). Nitrite: Nitrite consumption significantly increased with exercise, from 156 ± 59 pmol/ml/min/100 ml of tissue during L-NMMA infusion to 1583 ± 517 pmol/ml/min/100 ml of tissue during L-NMMA infusion with exercise, suggesting that intravascular nitrite is used even during physiological stress.