Figure 2.
Effect of histone H2A-mediated cytokine gene transfer on tumor cytolysis and IFN-γ production. Splenocytes of mice (n = 3) injected with 2 × 106 NXS2 cells genetically engineered to transiently produce scIL-12 (A and C) and IL-2 (B) were isolated 14 days after NXS2 cell injection and used in a standard 51Cr release assay against NXS2 target cells (A and B) or analyzed for the production of IFN-γ by ELISA (C). Each data point represents mean value ± SE. Differences between experimental groups and control groups were statistically significant (P < 0.005).