Figure 3  .

Photomicrographs demonstrating KDR immunostaining of non-diabetic retina (A), diabetic retina with PDR (B, C), the same retina stained with GFAP (D), and diabetic retina post laser but with no residual PDR (E). Immunostaining for KDR was greatest in the diabetic tissue with PDR and was minimal in most other diabetic tissue. Interestingly, immunostaining in the diabetic retinas which had undergone apparently successful laser treatment was reduced compared with the staining intensity in the retinas with PDR. Immunoreactivity for KDR was abolished in a control specimen of PDR retina processed with omission of the primary antibody (F). Magnification = A, C, D ×156; B ×60; E ×118; F ×78.