Figure 2.

Electron and light microscopic images of nanobacteria-like particles scraped from DMEM-containing subcultures of 0.45-μm membrane-filtered saliva. (a) SEM of biofilm material. (Bar = 5 μm.) (b) TEM of nanobacteria-like particles observed in thin sections of biofilm material. (Bar = 1 μm.) (c) TEM of negatively stained biofilm material showing small coccoid-shaped particles. (Bar = 0.2 μm.) (d) Light micrograph of biofilm material stained with Hoechst 33258 showing nonspecific surface fluorescence of coccoid-shaped particles. (Bar = 1 μm.)