Figure 1  .

Distribution and persistence of PS-ODNs (DS 012) in the retina 2 weeks after intravitreal injection (A-D). (A) 10.0 nmol, (B) retinal morphology of (A), counterstaining with haemotoxylin. The arrows indicate the RPE layer. (C) 2.0 nmol, the arrows are pointing to the nuclei of RPE cells. Inset: cytoplasmic distribution of PS-ODN in RPE observed by confocal laser scanning microscopy. (D) 0.5 nmol. (E and F) 5.0 nmol, 6 weeks (E) and 8 weeks (F) after injection. (G and H) Fluorescent microscopic images from the control eyes injected with fluorescent 6-FAM at 1 hour (G) and 24 (H) hours after injection. The fluorescent 6-FAM was mostly confined to the vitreous but some just entered into the ganglion cell layer at 1 hour after injection (G). However, no fluorescent signal was detected in the neural retina at 24 hours after injection (H). Original magnifications: (A-H) ×50; inset of (C) ×100. RPE = retinal pigment epithelium; POS = photoreceptor outer segment; ONL = outer nuclear layer; INL = inner nuclear layer; GCL = ganglion cell layer; ILM = inner limiting membrane.